《植物生理学报》 2009, 45(6): 571-574

光质对湛江等鞭金藻生长和脂肪酸组成的影响

柴雨, 吴垠*, 赵慧慧, 于丹

大连水产学院农业部海洋水产增养殖学与生物技术重点开放实验室, 辽宁大连116023

通信作者：吴垠；E-mail: wuyin@dlfu.edu.cn；Tel: 0411-83047135

摘　要：

在气升式光生物反应器中研究不同光质光影响湛江等鞭金藻的生长。结果表明, 藻细胞密度的大小顺序为: 红光>白 光 + 红光、白光>白光 + 蓝光 + 红光>白光 + 蓝光>蓝光。蓝光下多不饱和脂肪酸百分含量最高, 占总脂肪酸的 50.01%。白 光下总单不饱和脂肪酸和总饱和脂肪酸含量最高, 占总脂肪酸的24.19%和27.46%。多不饱和脂肪酸中C18:4n-3含量最高, 占 总脂肪酸的 20.3%~23.3%, 最高值出现在蓝光下; 其次为 C22:6n-3 (DHA), 占总脂肪酸的 10.2%~12.3%, 在蓝光和白光 + 蓝光 中较高; 而 C18:2n-6 和 C18:3n-3 均以红光下的为最高, 分别达 3.11% 和 8.04%。

关键词：湛江等鞭金藻; 光生物反应器; 光质; 生长; 脂肪酸

收稿：2008-12-22   修定：2009-05-04

资助：国家科技支撑项目(2006BAD09A12)。

Effects of Light Qualities on Growth and Fatty Acid Composition of Isochrysis zhanjiangensis Hu & Liu

CHAI Yu, WU Yin*, ZHAO Hui-Hui, YU Dan

Key Laboratory of Mariculture and Biotechnology of Agriculture Ministry, Dalian Fisheries University, Dalian, Liaoning 116023, China

Corresponding author: WU Yin; E-mail: wuyin@dlfu.edu.cn; Tel: 0411-83047135

Abstract:

The effects of different light qualities on the growth of Isochrysis zhanjiangensis in the air-lift photobioreactor systems were studied. The results indicated that, the cell density of I. zhanjiangensis showed that: red light> white+red light, white light>white+red+blue light>white+blue light>blue light. The content of total poly-unsaturated fatty acids reached the maximal value under blue light, accounted for 50.01% of total fatty acids. The content of total mono-unsaturated fatty acids and total saturated fatty acids reached the maximal value under white light, accounted for 24.19% and 27.46% of total fatty acids. Under blue light the C_18:4n-3 content was the highest in poly-unsaturated fatty acids, accounted for 20.3% to 23.3% of total fatty acids; and C_22:6n-3 (DHA) content followed, accounted for 10.2% to 12.3% of total fatty acids under blue and white+blue light; under red light C_18:2n-6 and C_18:3n-3 contents reached 3.11% and 8.04% respectively.

Key words: Isochrysis zhanjiangehsis; photobioreactor; light quality; growth; fatty acids